Supplier: SIGMA ALDRICH MICROSCOPY

Gram’s safranin solution, also known as Safranin T or Basic Red 2, is a ready-to-use staining solution that comprises 10% safranin in ethanol. Safranin is a basic, cationic, azine dye containing a planar, diaminophenazine chromophore, with a phenyl substituent attached to a ring nitrogen. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. It is most notably used in bacteriology as a counterstain in Gram staining to differentiate between Gram-positive and Gram-negative bacteria.

Supplier: Merck

Counterstain for Gram staining

Supplier: Merck

Gram-colour staining kit, containing 500 ml each of crystal violet solution, Lugol's solution stabilised, Safranin solution and 2×500 ml decolourisation solution.

Supplier: VWR Chemicals

Part Fleming Triple stain, a chromosome stain prepared with light green SF and in Gram stain.

Supplier: SIGMA ALDRICH MICROSCOPY

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in gram staining.

Supplier: SIGMA ALDRICH MICROSCOPY

Tissues are stained for Gram-positive organisms with crystal violet, mordanted in iodine, decolorized, stained for Gram-negative organisms with safranin O and then counterstained with tartrazine. For delineating Gram-positive and Gram-negative organisms

Supplier: SIGMA ALDRICH MICROSCOPY

Films are stained for Gram-positive organisms with crystal violet, mordanted in iodine, decolorized and then stained for Gram-negative organisms with safranin O. For delineating Gram-positive and Gram-negative organisms in films.

Supplier: VWR Chemicals

Safranine O solution

Supplier: RAL

Gram-Hücker staining is based on the same principle than the Gram-Nicolle staining. The structure of the bacterial walls and their permeability to alcohol help differentiate the Gram-negative bacteria from the Gram-positive ones. In this technique, Carbolic Gentian Violet is replaced by Crystal Violet Oxalate (that does not require filtration) and Ziehl-Carbolic Fuchsin by Safranin.

Supplier: SIGMA ALDRICH MICROSCOPY

Gram staining is one of the most significant staining techniques in microbiology. Gram′s decolouriser solution is a mixture of ethanol and acetone. Gram′s decolouriser solution is used in Gram staining procedure, after the iodine step to remove the crystal violet dye. The decolouriser dissolves the lipid layer from the gram-negative cells.

Supplier: Merck

Used for Gram staining

Supplier: VWR Chemicals

Gram's decolourising solution contains acetone and ethanol

Supplier: SIGMA ALDRICH MICROSCOPY

Used as a kit component in Gram Stain Procedure (Sigma Procedure HT90). Iodine, 0.33%, and potassium iodide, 0.66%.

Supplier: MP Biomedicals

Malachite green is use as counter-stain against fuchsin which stains gram-positive and gram-negative bacteria reddish colors and safranin which stains nuclei red. Malachite green stain background to the surrounding tissue blue-green.

Supplier: SIGMA ALDRICH MICROSCOPY

Gram’s iodine solution is used as a mordant in the Gram staining process. It is added after the primary staining step with crystal violet; the resultant crystal violet - Iodine complex imparts purple color to the cells.

Supplier: VWR Chemicals

Gram's decolourising solution

Supplier: SIGMA ALDRICH MICROSCOPY

May contain minor quantities of precipitated dye.

Supplier: VWR Chemicals

Gram's decolourising solution contains acetone and 2-propanol

Supplier: Merck

Lugol’s solution stabilized with PVP is used in bacteriology, for example, for the staining of Gram bacteria

Supplier: Merck

Diluted iodine-potassium iodide solution used to form a crystal violet dye-iodine complex in Gram staining.

Supplier: SIGMA ALDRICH MICROSCOPY

Lugol′s solution is a ready-to-use solution that comprises 6% iodine in 4% potassium iodide. It is commonly used as a mordant for Gram staining in bacteriology. It is used for staining bacterial target structures for diagnostic purposes by fixing, staining, counterstaining, and mounting bacteriological specimen materials.

Supplier: SIGMA ALDRICH MICROSCOPY

Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.

Supplier: Merck

The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.

Supplier: SIGMA ALDRICH MICROSCOPY

Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.
Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.

Supplier: VWR Chemicals

VWR® penicillin-streptomycin solution (100X) is a large spectrum antibiotic designed to control and destroy gram-negative and gram-positive bacteria.

Supplier: VWR Chemicals

Staining solutions used in histology and cytology for the counterstaining of the cell nuclei red. Classically in a Gram staining and endospore staining.

Supplier: Biowest

Penicillin-Streptomycin acts against Gram-negative and Gram-positive bacteria.

Supplier: Biotium

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.

Supplier: SIGMA ALDRICH MICROSCOPY

Safranine O, Sigma-Aldrich®

Supplier: Biowest

Penicillin-Streptomycin acts against gram-negative, gram-positive bacteria and mycoplasma.