VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.
- Most suitable choice for routine applications where high fidelity is not required
- High performance, thermostable DNA polymerase
- Optimal for TA cloning
VWR® Taq DNA Polymerase Glycerol Free has both a 5'→3' DNA polymerase and a 5'→3' exonuclease activity. The enzyme lacks a 3'→5' exonuclease activity (no proofreading ability). VWR® Taq DNA Polymerase Glycerol Free leaves an A' overhang, which makes the enzyme ideal for TA cloning.
10X Key Buffer: Tris-HCL pH 8,5, (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween-20®
10X Extra Buffer: 100 mM Tris-HCL pH 8,3, 500 mM KCl, 15 mM MgCl₂, 1% Triton X-100
10X Mg-Free Key Buffer: Tris-HCL pH 8,5, (NH₄)₂SO₂, 1% Tween-20®
10X Mg-Free Extra Buffer: Tris-HCL pH 8,3, KCl, 1% Triton X-100
- Application:Freeze-dryingStandard amplification
- Format:Enzyme with/without buffer
- High fidelity:No
- Hot start:No
- Storage conditions:– 20 °C (long term), + 4 °C (6 months)
- Amplicon size:≤ 5kb
- Processivity:Standard
- Exonuclease activity:5’→3’
- Product overhang:3'A
- Features:Glycerol free
- Buffer composition:25 mM MgCl2
- Concentration:5 U/µl
- Description:Taq DNA Polymerase Glycerol Free 5U/µl 5.000 units
- Size:10 x 0.1 ml
- Units:5000
Related Information
Certificates
Search by Lot NumberFast PCR protocols for VWR® Taq DNA Polymerase Versatile VWR PCR enzymes VWR Taq DNA polymerase is more thermo-stable than most people think VWR TEMPase Hot Start DNA polymerase - for robust and reliable PCR
- Pk
- Taq DNA Polymerase, glycerol-free, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂Taq DNA Polymerase, glycerol-free, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂1.000 EU1 * 1.000 EU£192.00
- Taq DNA Polymerase, glycerol-free, without Key or Extra Buffer, 25 mM MgCl₂Taq DNA Polymerase, glycerol-free, without Key or Extra Buffer, 25 mM MgCl₂733-19995.000 EU1 * 5.000 EU£692.00
- Taq DNA Polymerase Glycerol Free 5U/µl 100.000 units
- Taq DNA Polymerase, glycerol-free, without Key or Extra Buffer, 25 mM MgCl₂, 200 000 unitsTaq DNA Polymerase, glycerol-free, without Key or Extra Buffer, 25 mM MgCl₂, 200 000 units1 KIT1 * 1 KIT
- Taq DNA Polymerase Glycerol Free 5U/µl 375.000 units