Antibody clean up kit, Pearl™

Supplier: G-Biosciences
Pearl™
786-803EA 613 GBP
786-803
Antibody clean up kit, Pearl™
Protein Purification Protein Purification Kits
The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

Purified and commercial antibodies are routinely stored in buffers containing bovine serum albumin (BSA) and gelatin that act as stabilisers during long term storage. In routine applications, such as ELISA, Western blotting and other immunodetection techniques, these proteins generally do not interfere. The presence of the protein stabilisers do interfere with antibody labelling and conjugation techniques, including biotinylation, fluorescent dye labelling, covalent antibody immobilisation and antibody fragmentation experiments.
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