About this item
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
Denhardt solution is suitable for nucleic acid hybridisation. Denhardt's solution is a mi×ture of high-molecular weight polymers capable of saturating non-specific binding sites and artificially increasing the concentration of available probe. It consists of 2% Ficoll 400, 300mM NaCl, 2% polyvinylpyrrolidone, and 2% BSA.
The SSPE or or Saline-Sodium phosphate-EDTA buffer is a general hybridisation buffer that consists of 0,2 M phosphate buffer (at a pH of 7,4), 2,98 M NaCl, and 0,02 M EDTA.
The TMAC hybridisation Solution consists of 3 M Tetramethyl ammonium chloride, 50mm Tris-HCl (at a pH of 8,0), 1 mm EDTA (at a pH of 8,0), and 0,1% SDS.
The TNT Buffer is a general molecular biology and hybridisation buffer that consists of 0,1 M Tris-HCl (at a pH of 7,5), 0,15 M NaCl, and 0,05% Tween®-20.
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Product Information
- Catalog #Supplier #PkAvailabilityPrice
- Church & Gilbert's [2X] bufferChurch & Gilbert's [2X] buffer786-558786-558250 mL1 * 250 mL£177.00Supplier Article Number: 786-558Local Article Number: GENO786-558
- Church & Gilbert's [2X] bufferChurch & Gilbert's [2X] buffer786-559786-559500 mL1 * 500 mL£299.00Supplier Article Number: 786-559Local Article Number: GENO786-559
- Denhardt solution [100X]Denhardt solution [100X]786-525786-52550 mL1 * 50 mL£265.00Supplier Article Number: 786-525Local Article Number: GENO786-525
- TMAC Hyb Solution [3X]TMAC Hyb Solution [3X]786-538786-5381 L1 * 1 L£276.00Supplier Article Number: 786-538Local Article Number: GENO786-538
- TNT BufferTNT Buffer786-470786-470100 mL1 * 100 mL£120.00Supplier Article Number: 786-470Local Article Number: GENO786-470
- TNT BufferTNT Buffer786-471786-471500 mL1 * 500 mL£272.00Supplier Article Number: 786-471Local Article Number: GENO786-471
- EKONO® hybridization bufferEKONO® hybridization buffer786-160786-1601 L1 * 1 L£276.00Supplier Article Number: 786-160Local Article Number: GENO786-160
- Hybridization Solution I (0.9M NaCl, 90mM Trisodium citrate, 5% Tween® 20)Hybridization Solution I (0.9M NaCl, 90mM Trisodium citrate, 5% Tween® 20)786-528786-5281 L1 * 1 L£168.00Supplier Article Number: 786-528Local Article Number: GENO786-528
- Hybridization Denaturing Solution (1.5M NaCl, 0.5N NaOH)Hybridization Denaturing Solution (1.5M NaCl, 0.5N NaOH)R015R0151 L1 * 1 L£139.00Supplier Article Number: R015Local Article Number: GENOR015
- Hybridization Denaturing Solution (1.5M NaCl, 0.5N NaOH)Hybridization Denaturing Solution (1.5M NaCl, 0.5N NaOH)R016R0164 L1 * 4 L£221.00Supplier Article Number: R016Local Article Number: GENOR016
- Hybridization Neutralizing Solution (1M Tris.HCl (pH 7.4), 1.5M NaCl)Hybridization Neutralizing Solution (1M Tris.HCl (pH 7.4), 1.5M NaCl)R017R0171 L1 * 1 L£156.00Supplier Article Number: R017Local Article Number: GENOR017
- Hybridization Neutralizing Solution (1M Tris.HCl (pH 7.4), 1.5M NaCl)Hybridization Neutralizing Solution (1M Tris.HCl (pH 7.4), 1.5M NaCl)R018R0184 L1 * 4 L£298.00Supplier Article Number: R018Local Article Number: GENOR018
- SSPE buffer, 20XSSPE buffer, 20X786-024786-024500 mL1 * 500 mL£66.00Supplier Article Number: 786-024Local Article Number: GENO786-024
- SSPE buffer, 20XSSPE buffer, 20XR021R0211 L1 * 1 L£101.00Supplier Article Number: R021Local Article Number: GENOR021
- SSPE buffer, 20XSSPE buffer, 20XR022R0224 L1 * 4 L£263.00Supplier Article Number: R022Local Article Number: GENOR022